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Gene expression levels were determined relative to a reference using the RQMAX method, and the results were presented on a logarithmic scale

To prepare membranes containing either FLVCR2 or PNS1 protein, 300 g of cells was thawed and resuspended in 750 ml lysis buffer (600 mM NaCl, 100 mM TrisHCl, pH 7.5), supplemented with 1 mM PMSF, followed by 5 1 min cycles of bead beating with 0.5-mm glass beads (BioSpec Products)

a warm bath can have a similar effect

doi: 10.1089/thy.2020.0299 90 FaamBGhadiriAAGhaffariMATotonchiMKhorsandiL